pDS24
(Plasmid #58952)

• Purpose: Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
• Depositing Lab: Thomas Fox
• Publication: Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93(11):5253-7.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 8050

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mitochondrially coded Arg8 protein
• Alt name: acetylornithine aminotransferase
• Alt name: ARG8m
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2042
• Mutation: Coding sequence synthetic in S. cerevisiae mitochondrial genetic code
• GenBank ID: U31093.1
• Promoter: n/a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: SacI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Insert also contains an internal SacI site.