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pAAV-Syn-ChrimsonR-tdT
(Plasmid #59171)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59171 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV
  • Backbone size w/o insert (bp) 4368
  • Total vector size (bp) 6864
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C (use ampicillin if using DH5a)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ChrimsonR-tdTomato
  • Alt name
    Chrimson K176R mutant
  • Alt name
    ChR88m19-tdTomato
  • Species
    Chlamydomonas noctigama
  • Insert Size (bp)
    2496
  • GenBank ID
    KF992060
  • Promoter Syn
  • Tag / Fusion Protein
    • tdTomato (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CTGCGTATGAGTGCAAG
  • 3′ sequencing primer cagcgtatccacatagcg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Plasmid is completely sequenced by the depositing lab except for part of the 3' ITR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Syn-ChrimsonR-tdT was a gift from Edward Boyden (Addgene plasmid # 59171)
  • For your References section:

    Independent optical excitation of distinct neural populations. Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, Boyden ES. Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9. 10.1038/nmeth.2836 PubMed 24509633