pLL3.7 Alk.2 shRNA
(Plasmid #59297)

• Purpose: shRNA to mouse Alk
• Depositing Lab: Ulrike Heberlein
• Publication: Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41. doi: 10.1523/JNEUROSCI.3415-11.2011.

Backbone

• Vector backbone: pLL3.7
• Backbone manufacturer: Addgene Plasmid #11795
• Backbone size w/o insert (bp): 7650
• Total vector size (bp): 7579
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: Although this plasmid grows in standard bacteria, to reduce the chance of recombination we recommend that you grow the plasmid at 30oC in Stbl3 cells from Invitrogen.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alk.2 shRNA
• Alt name: Alk.2
• gRNA/shRNA sequence: GCAACGCAGCATCAAACAA
• Species: M. musculus (mouse)
• Promoter: mouse U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: U6 (5'-cagtgcaggggaaagaatagtagac-3')

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Luk Parijs
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.