GFP-FKBP-LCa
(Plasmid #59353)

• Purpose: Expresses human clathrin light chain A tagged with GFP & FKBP in mammalian cells.
• Depositing Lab: Stephen Royle
• Publication: Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4676
• Total vector size (bp): 5781
• Modifications to backbone: Insertion of FKBP domain to tag gene of interest at N-terminus.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Clathrin Light Chain A
• Alt name: LCa
• Alt name: CLTA
• Alt name: non-neuronal isoform
• Species: H. sapiens (human)
• Insert Size (bp): 657
• GenBank ID: NM_001833.3
• Entrez Gene: CLTA (a.k.a. LCA)
• Tags / Fusion Proteins:
  • EGFP (N terminal on backbone)
  • FKBP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: - (unknown if destroyed)
• 3′ cloning site: Acc65I (unknown if destroyed)
• 5′ sequencing primer: pEGFP-C2_FP
• 3′ sequencing primer: pEGFP-C2_RP

Resource Information

• A portion of this plasmid was derived from a plasmid made by: LCa: IMAGE 3944942
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry