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pBrain-GFP-FKBP-TACC3-shTACC3
(Plasmid #59354)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59354 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBrain
  • Backbone manufacturer
    Stephen J Royle
  • Backbone size w/o insert (bp) 5816
  • Total vector size (bp) 8330
  • Modifications to backbone
    Includes EGFP and FKBP to tag protein of interest at N-terminus.
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Transforming Acidic Coiled Coil protein 3
  • Alt name
    TACC3
  • Alt name
    ERIC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2514
  • GenBank ID
    NM_006342.2
  • Entrez Gene
    TACC3 (a.k.a. ERIC-1, ERIC1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • FKBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBrain-GFP-FKBP-TACC3-shTACC3 was a gift from Stephen Royle (Addgene plasmid # 59354 ; http://n2t.net/addgene:59354 ; RRID:Addgene_59354)
  • For your References section:

    Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ. J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26. 10.1242/jcs.124834 PubMed 23532825