pET23a_mod_RBPMS2_FL
(Plasmid
#59394)
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PurposePlasmid for bacterial overexpression of RBPMS2
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepet23a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3494
- Total vector size (bp) 4352
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRBPMS2
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SpeciesH. sapiens (human)
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Insert Size (bp)858
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GenBank IDNM_194272.1
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Entrez GeneRBPMS2
- Promoter T7
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Tags
/ Fusion Proteins
- T7 (N terminal on backbone)
- FLAG (N terminal on backbone)
- HA (N terminal on backbone)
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7_promoter_primer
- 3′ sequencing primer T7_Terminal_Primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET23a_mod_RBPMS2_FL was a gift from Thomas Tuschl (Addgene plasmid # 59394 ; http://n2t.net/addgene:59394 ; RRID:Addgene_59394) -
For your References section:
Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets. Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, Larsson E, Ohler U, Tuschl T. RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014 May 23. 10.1261/rna.045005.114 PubMed 24860013
