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pLV-Enh eGFP Reporter-huKC2-IGR21
(Plasmid #59456)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59456 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Brachyury-eGFP
  • Backbone size w/o insert (bp) 7967
  • Total vector size (bp) 8656
  • Modifications to backbone
    Original vector is Brachyury-eGFP (Kita-Matsuo et al. 2009) with the Brachyury promoter cloned out and replaced with the minimal Hsp68 promoter from the Hsp68-lacZ-Gateway plasmid (Pennacchio et al. 2006)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    huKrt1 3’intergenic region
  • Alt name
    hg18 chr12: 51342842-51343530
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    689
  • Promoter Mu Hsp68 minimal promoter

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CGATCCTGTAGTTGTAACCATTCATTATTAG
  • 3′ sequencing primer CCCAGCCCCGCAGCT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-Enh eGFP Reporter-huKC2-IGR21 was a gift from Benjamin Yu (Addgene plasmid # 59456 ; http://n2t.net/addgene:59456 ; RRID:Addgene_59456)