Nanog-CreER targeting construct
PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locus
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59720||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 15024
Vector typeMouse Targeting
Selectable markersHygromycin ; DT
Growth in Bacteria
Copy numberHigh Copy
- Promoter mNanog
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TCTTTCTGTGGGAAGGCTGC (Common Sequencing Primers)
The backbone contains DT gene for negative selection.
Please visit https://www.biorxiv.org/content/early/2014/08/23/008284 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Nanog-CreER targeting construct was a gift from Jacob Hanna (Addgene plasmid # 59720 ; http://n2t.net/addgene:59720 ; RRID:Addgene_59720)
For your References section:Transient acquisition of pluripotency during somatic cell transdifferentiation with iPSC reprogramming factors. Maza I, Caspi I, Zviran A, Chomsky E, Rais Y, Viukov S, Geula S, Buenrostro JD, Weinberger L, Krupalnik V, Hanna S, Zerbib M, Dutton JR, Greenleaf WJ, Massarwa R, Novershtern N, Hanna JH. Nat Biotechnol. 2015 Jun 22. doi: 10.1038/nbt.3270. 10.1038/nbt.3270 PubMed 26098448