pSA110
(Plasmid #59784)

• Purpose: drives mCherry in C. elegans intestinal cells
• Depositing Lab: Jeremy Nance
• Publication: Armenti et al Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 2014 Nov 5.

Backbone

• Vector backbone: pCFJ150
• Total vector size (bp): 8283
• Modifications to backbone: modified to include PmeI restriction digest site for Gibson cloning inserts
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: elt-2 promoter
• Species: C. elegans (nematode)
• Insert Size (bp): 5002
• Entrez Gene: elt-2 (a.k.a. CELE_C33D3.1)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ggtcgagctgaatacacgtgc
• 3′ sequencing primer: TCTATAATCTATTTTCTAGTTTCTATTTTATTAGAATGC

Gene/Insert 2

• Gene/Insert name: mCherry (worm)
• Species: C. elegans (nematode)
• Insert Size (bp): 864

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATGGTCTCAAAGGGTGAAGAAG
• 3′ sequencing primer: CTACTTATACAATTCATCCATGCCAC

Gene/Insert 3

• Gene/Insert name: unc-54 3' UTR
• Insert Size (bp): 705

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gtccaattactcttcaacatccc
• 3′ sequencing primer: AAACAGTTATGTTTGGTATATTGGGAATG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry