pJN601
(Plasmid #59790)

• Purpose: zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker
• Depositing Lab: Jeremy Nance
• Publication: Armenti et al Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 2014 Nov 5.

Backbone

• Vector backbone: pBR322
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 6800
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ZF1 domain from pie-1
• Species: C. elegans (nematode)
• Insert Size (bp): 108

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: custom
• 3′ sequencing primer: custom

Gene/Insert 2

• Gene/Insert name: N-terminal half of yfp with introns
• Species: jellyfish
• Insert Size (bp): 375

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: custom
• 3′ sequencing primer: custom

Gene/Insert 3

• Gene/Insert name: intron containing floxed unc-119 gene in reverse orientation
• Species: C. elegans (nematode); Bateriophage P1
• Insert Size (bp): 2748

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: custom
• 3′ sequencing primer: custom

Gene/Insert 4

• Gene/Insert name: C-terminal half of yfp with introns
• Species: jellyfish
• Insert Size (bp): 443

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: custom
• 3′ sequencing primer: custom

Resource Information

• Supplemental Documents:
  • pJN601_genbank
• A portion of this plasmid was derived from a plasmid made by: yfp with introns derived from plasmid pPD134.99, a gift from Andrew Fire.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry