Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pLT 653
(Plasmid #59996)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59996 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    L4440
  • Backbone manufacturer
    Andrew Fire
  • Backbone size w/o insert (bp) 2790
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Utilize HT115(DE3) cells for induction of dsRNA
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    dhc-3
  • Alt name
    B0365.7
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    465
  • Mutation
    partial genomic
  • GenBank ID
    NM_073871
  • Entrez Gene
    dhc-3 (a.k.a. CELE_B0365.7)

Gene/Insert 2

  • Gene/Insert name
    klp-16
  • Alt name
    C41G7.2
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1170
  • Mutation
    partial genomic
  • GenBank ID
    NM_060126
  • Entrez Gene
    klp-16 (a.k.a. CELE_C41G7.2)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Source BioScience LifeSciences C. elegans RNAi library clones utilized in plasmid construction (Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M. and Ahringer, J. (2000) Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325–330.)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLT 653 was a gift from Lisa Timmons (Addgene plasmid # 59996 ; http://n2t.net/addgene:59996 ; RRID:Addgene_59996)
  • For your References section:

    Systematic comparison of bacterial feeding strains for increased yield of Caenorhabditis elegans males by RNA interference-induced non-disjunction. Timmons L, Luna H, Martinez J, Moore Z, Nagarajan V, Kemege JM, Asad N. FEBS Lett. 2014 Jul 24. pii: S0014-5793(14)00572-9. doi: 10.1016/j.febslet.2014.07.023. 10.1016/j.febslet.2014.07.023 PubMed 25066299