pΔΦ
(Plasmid #60009)

• Purpose: (Empty Backbone) Deleted foamy virus vector plasmid containing a backbone with a polylinker region for insertion of a transgene cassette. Ampicillin resistant.
• Depositing Lab: David Russell
• Publication: Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Backbone

• Vector backbone: pΔΦ is a deleted foamy vector pHSRV13
• Backbone manufacturer: R. M. Flugel 1991
• Backbone size (bp): 3800
• Modifications to backbone: pΔΦ has deletions in the gag, pol, env, and bel1–3 accessory genes, as well as the LTR U3 region, but retains an essential 2.5-kb cis-acting region. In addition, a polylinker region to insert a transgene cassette, and stop codons were introduced into the remaining gag sequences to prevent expression of viral peptides and to eliminate dominant-negative effects of a Gag-Pol fusion protein.
• Vector type: deleted foamy virus
• Promoter: CMV
• Selectable markers: none; to be added in cassette

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha ; DH10B
• Copy number: High Copy

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • Protocol for ΔΦ FV Vector Production
  • Annotated Sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ΔΦ vectors are “”Deleted Foamy” virus vectors that only retain essential cis-acting viral sequences. The 4 plasmids required to make ΔΦ vectors are pCiES, pCiGSΔΨ, pCiPS, and pΔΦ. The first three plasmids are helper constructs expressing Env, Gag, and Pol respectively. pΔΦ is a deleted foamy vector backbone with a polylinker for you to insert a transgene cassette. A description of the plasmids has been published (Trobridge et al., 2002).

For additional information, please see:
http://depts.washington.edu/dwrlab/reagents/foamy-virus-reagents/
http://depts.washington.edu/dwrlab/reagents/aav-serotype-reagents/