Human _TFAM_NoMTS_Dimer_pET28a+
(Plasmid #60013)

• Purpose: Expresses TFAM No MTS dimerization mutant in bacterial cells
• Depositing Lab: David Chan
• Publication: Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/ncomms4077.

Backbone

• Vector backbone: pET28a+
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 5953
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human TFAM No MTS
• Alt name: TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
• Species: H. sapiens (human)
• Insert Size (bp): 618
• Mutation: Missing 1st 42 aas (Mitochondrial targeting sequence); Changed K95A, Y99F, E106A, E112A and R116A
• GenBank ID: 7019
• Entrez Gene: TFAM (a.k.a. MTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that depositor's assembled sequence is WT TFAM; mutations are clear in Addgene quality control sequence.