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pOTTC293 - pAAV EF1a V5-synuclein (WT)
(Plasmid #60057)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60057 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (Addgene 20297)
  • Backbone manufacturer
    Karl Deisseroth
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-synuclein
  • Alt name
    SCNA
  • Species
    H. sapiens (human); human
  • Insert Size (bp)
    423
  • GenBank ID
    NM_000345
  • Entrez Gene
    SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
  • Promoter EF1a
  • Tag / Fusion Protein
    • V5 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EF1a_F1
  • 3′ sequencing primer WPRE_R1
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC293 - pAAV EF1a V5-synuclein (WT) was a gift from Brandon Harvey (Addgene plasmid # 60057 ; http://n2t.net/addgene:60057 ; RRID:Addgene_60057)
  • For your References section:

    The beneficial effect of a prolyl oligopeptidase inhibitor, KYP-2047, on alpha-synuclein clearance and autophagy in A30P transgenic mouse. Savolainen MH, Richie CT, Harvey BK, Mannisto PT, Maguire-Zeiss KA, Myohanen TT. Neurobiol Dis. 2014 Aug;68:1-15. doi: 10.1016/j.nbd.2014.04.003. Epub 2014 Apr 16. 10.1016/j.nbd.2014.04.003 PubMed 24746855