Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pLL7.0: tgRFPt-SSPB WT Citations (16)

Originally described in: Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins.
Guntas G, Hallett RA, Zimmerman SP, Williams T, Yumerefendi H, Bear JE, Kuhlman B Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):112-7. doi: 10.1073/pnas.1417910112. Epub 2014 Dec 22.
PubMed Journal

Articles Citing pLL7.0: tgRFPt-SSPB WT

Articles
Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Zimmerman SP, Hallett RA, Bourke AM, Bear JE, Kennedy MJ, Kuhlman B. Biochemistry. 2016 Sep 20;55(37):5264-71. doi: 10.1021/acs.biochem.6b00529. Epub 2016 Sep 8. PubMed
Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T. Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559. PubMed

Associated Plasmids

Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. Milas A, Jagric M, Martincic J, Tolic IM. Methods Cell Biol. 2018;145:191-215. doi: 10.1016/bs.mcb.2018.03.024. Epub 2018 Apr 26. PubMed
Discovering Selective Binders for Photoswitchable Proteins Using Phage Display. Reis JM, Xu X, McDonald S, Woloschuk RM, Jaikaran ASI, Vizeacoumar FS, Woolley GA, Uppalapati M. ACS Synth Biol. 2018 Oct 19;7(10):2355-2364. doi: 10.1021/acssynbio.8b00123. Epub 2018 Sep 27. PubMed

Associated Plasmids

Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher JE, Brangwynne CP. Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 10.1016/j.cell.2018.10.048. PubMed

Associated Plasmids

Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP. Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057. PubMed

Associated Plasmids

The activity of Sac1 across ER-TGN contact sites requires the four-phosphate-adaptor-protein-1. Venditti R, Masone MC, Rega LR, Di Tullio G, Santoro M, Polishchuk E, Serrano IC, Olkkonen VM, Harada A, Medina DL, La Montagna R, De Matteis MA. J Cell Biol. 2019 Mar 4;218(3):783-797. doi: 10.1083/jcb.201812021. Epub 2019 Jan 18. PubMed
A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Liu Q, Sinnen BL, Boxer EE, Schneider MW, Grybko MJ, Buchta WC, Gibson ES, Wysoczynski CL, Ford CP, Gottschalk A, Aoto J, Tucker CL, Kennedy MJ. Neuron. 2019 Jan 15. pii: S0896-6273(19)30003-0. doi: 10.1016/j.neuron.2019.01.002. PubMed

Associated Plasmids

Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. Inaba H, Miao Q, Nakata T. J Biol Chem. 2021 Jan-Jun;296:100290. doi: 10.1016/j.jbc.2021.100290. Epub 2021 Jan 13. PubMed

Associated Plasmids

Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces. Jagric M, Risteski P, Martincic J, Milas A, Tolic IM. Elife. 2021 Jan 22;10. pii: 61170. doi: 10.7554/eLife.61170. PubMed
Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment. Natwick DE, Collins SR. ACS Synth Biol. 2021 May 21;10(5):1009-1023. doi: 10.1021/acssynbio.0c00511. Epub 2021 Apr 12. PubMed

Associated Plasmids

VPS13D bridges the ER to mitochondria and peroxisomes via Miro. Guillen-Samander A, Leonzino M, Hanna MG, Tang N, Shen H, De Camilli P. J Cell Biol. 2021 May 3;220(5). pii: 212021. doi: 10.1083/jcb.202010004. PubMed

Associated Plasmids

The phosphoinositide coincidence detector Phafin2 promotes macropinocytosis by coordinating actin organisation at forming macropinosomes. Schink KO, Tan KW, Spangenberg H, Martorana D, Sneeggen M, Stevenin V, Enninga J, Campsteijn C, Raiborg C, Stenmark H. Nat Commun. 2021 Nov 12;12(1):6577. doi: 10.1038/s41467-021-26775-x. PubMed
Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. Yamamoto K, Miura H, Ishida M, Mii Y, Kinoshita N, Takada S, Ueno N, Sawai S, Kondo Y, Aoki K. Nat Commun. 2021 Dec 8;12(1):7145. doi: 10.1038/s41467-021-27458-3. PubMed

Associated Plasmids

Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease. Li X, Zhang Y, Xu L, Wang A, Zou Y, Li T, Huang L, Chen W, Liu S, Jiang K, Zhang X, Wang D, Zhang L, Zhang Z, Zhang Z, Chen X, Jia W, Zhao A, Yan X, Zhou H, Zhu L, Ma X, Ju Z, Jia W, Wang C, Loscalzo J, Yang Y, Zhao Y. Cell Metab. 2023 Jan 3;35(1):200-211.e9. doi: 10.1016/j.cmet.2022.10.002. Epub 2022 Oct 28. PubMed
Engineering of bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s. Jang J, Tang K, Youn J, McDonald S, Beyer HM, Zurbriggen MD, Uppalapati M, Woolley GA. Nat Methods. 2023 Feb 23. doi: 10.1038/s41592-023-01764-8. PubMed

Associated Plasmids

If you have published an article using this material, please email us at [email protected] to have your article added to this page.