pCR
(Plasmid
#60619)
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Purpose(Empty Backbone) crRNA expression plasmid on pRS423 backbone
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS423
- Backbone size (bp) 5000
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Vector typeYeast Expression, CRISPR
- Promoter SNR52
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Feng Zhang
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR was a gift from Huimin Zhao (Addgene plasmid # 60619 ; http://n2t.net/addgene:60619 ; RRID:Addgene_60619) -
For your References section:
Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae. Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H. ACS Synth Biol. 2014 Sep 19. 10.1021/sb500255k PubMed 25207793
