Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed November 28th & 29th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of November 25th - 29th. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

BlastR-P2A-DD-TCOF1-donor
(Plasmid #60748)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60748 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PCR2.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3900
  • Total vector size (bp) 6300
  • Vector type
    Targeting donor
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TCOF1
  • Alt name
    Treacle
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2387
  • Entrez Gene
    TCOF1 (a.k.a. MFD1, TCS, TCS1, treacle)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BlastR-P2A-DD-TCOF1-donor was a gift from Benhur Lee (Addgene plasmid # 60748 ; http://n2t.net/addgene:60748 ; RRID:Addgene_60748)
  • For your References section:

    CRISPR/Cas9 allows efficient and complete knock-in of a destabilization domain-tagged essential protein in a human cell line, allowing rapid knockdown of protein function. Park A, Won ST, Pentecost M, Bartkowski W, Lee B. PLoS One. 2014 Apr 17;9(4):e95101. doi: 10.1371/journal.pone.0095101. eCollection 2014. 10.1371/journal.pone.0095101 PubMed 24743236