pESC-LEU-HsAIDSc
(Plasmid #60810)

• Purpose: to express human AID recoded for yeast codon usage
• Depositing Labs: Thomas Kunkel, Youri Pavlov
• Publication: Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

Backbone

• Vector backbone: pESC-LEU
• Backbone manufacturer: Stratagene
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AID
• Alt name: activation-induced cytidine deaminase
• Species: H. sapiens (human)
• Mutation: codon optimized for yeast
• Entrez Gene: AICDA (a.k.a. AID, ARP2, CDA2, HEL-S-284, HIGM2)
• Promoter: GAL1
• Tag / Fusion Protein:
  • Myc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GAL1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry