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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS Citations (46)

Originally described in: A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PubMed Journal

Articles Citing pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Articles
CRISPR/dCas9-mediated Transcriptional Inhibition Ameliorates the Epigenetic Dysregulation at D4Z4 and Represses DUX4-fl in FSH Muscular Dystrophy. Himeda CL, Jones TI, Jones PL. Mol Ther. 2015 Nov 3. doi: 10.1038/mt.2015.200. PubMed
CRISPR Technology for Genome Activation and Repression in Mammalian Cells. Du D, Qi LS. Cold Spring Harb Protoc. 2016 Jan 4;2016(1):pdb.prot090175. doi: 10.1101/pdb.prot090175. PubMed
Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter. Ji H, Jiang Z, Lu P, Ma L, Li C, Pan H, Fu Z, Qu X, Wang P, Deng J, Yang X, Wang J, Zhu H. Mol Ther. 2016 Mar;24(3):508-21. doi: 10.1038/mt.2016.7. Epub 2016 Jan 18. PubMed
Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed
Comparison of Cas9 activators in multiple species. Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871. PubMed

Associated Plasmids
Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems. Bialek JK, Dunay GA, Voges M, Schafer C, Spohn M, Stucka R, Hauber J, Lange UC. PLoS One. 2016 Jun 24;11(6):e0158294. doi: 10.1371/journal.pone.0158294. eCollection 2016. PubMed
The methyltransferase SETDB1 regulates a large neuron-specific topological chromatin domain. Jiang Y, Loh YE, Rajarajan P, Hirayama T, Liao W, Kassim BS, Javidfar B, Hartley BJ, Kleofas L, Park RB, Labonte B, Ho SM, Chandrasekaran S, Do C, Ramirez BR, Peter CJ, C W JT, Safaie BM, Morishita H, Roussos P, Nestler EJ, Schaefer A, Tycko B, Brennand KJ, Yagi T, Shen L, Akbarian S. Nat Genet. 2017 Aug;49(8):1239-1250. doi: 10.1038/ng.3906. Epub 2017 Jul 3. PubMed
Specific Expression of Interferon-gamma Induced by Synergistic Activation Mediator-Derived Systems Activates Innate Immunity and Inhibits Tumorigenesis. Liu S, Yu X, Wang Q, Liu Z, Xiao Q, Hou P, Hu Y, Hou W, Yang Z, Guo D, Chen S. J Microbiol Biotechnol. 2017 Oct 28;27(10):1855-1866. doi: 10.4014/jmb.1705.05081. PubMed
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed
DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A. Huang YH, Su J, Lei Y, Brunetti L, Gundry MC, Zhang X, Jeong M, Li W, Goodell MA. Genome Biol. 2017 Sep 18;18(1):176. doi: 10.1186/s13059-017-1306-z. PubMed

Associated Plasmids
Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila. Qian Y, Cao Y, Deng B, Yang G, Li J, Xu R, Zhang D, Huang J, Rao Y. Elife. 2017 Oct 6;6. pii: 26519. doi: 10.7554/eLife.26519. PubMed
TUBB3 overexpression has a negligible effect on the sensitivity to taxol in cultured cell lines. Tame MA, Manjon AG, Belokhvostova D, Raaijmakers JA, Medema RH. Oncotarget. 2017 May 10;8(42):71536-71547. doi: 10.18632/oncotarget.17740. eCollection 2017 Sep 22. PubMed
Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14. PubMed

Associated Plasmids
Co-optation of Tandem DNA Repeats for the Maintenance of Mesenchymal Identity. Balestrieri C, Alfarano G, Milan M, Tosi V, Prosperini E, Nicoli P, Palamidessi A, Scita G, Diaferia GR, Natoli G. Cell. 2018 May 17;173(5):1150-1164.e14. doi: 10.1016/j.cell.2018.03.081. Epub 2018 Apr 26. PubMed
BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Jiang W, Feng S, Huang S, Yu W, Li G, Yang G, Liu Y, Zhang Y, Zhang L, Hou Y, Chen J, Chen J, Huang X. Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4. PubMed

Associated Plasmids
A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. PubMed

Associated Plasmids
Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. PubMed

Associated Plasmids
The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Heurtier V, Owens N, Gonzalez I, Mueller F, Proux C, Mornico D, Clerc P, Dubois A, Navarro P. Nat Commun. 2019 Mar 7;10(1):1109. doi: 10.1038/s41467-019-09041-z. PubMed

Associated Plasmids
Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays. Kunii A, Hara Y, Takenaga M, Hattori N, Fukazawa T, Ushijima T, Yamamoto T, Sakuma T. CRISPR J. 2018 Oct;1:337-347. doi: 10.1089/crispr.2018.0009. PubMed
Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors. Tran NT, Bashir S, Li X, Rossius J, Chu VT, Rajewsky K, Kuhn R. Front Genet. 2019 Apr 30;10:365. doi: 10.3389/fgene.2019.00365. eCollection 2019. PubMed
In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Peter CJ, Saito A, Hasegawa Y, Tanaka Y, Nagpal M, Perez G, Alway E, Espeso-Gil S, Fayyad T, Ratner C, Dincer A, Gupta A, Devi L, Pappas JG, Lalonde FM, Butman JA, Han JC, Akbarian S, Kamiya A. Nat Commun. 2019 Sep 11;10(1):4112. doi: 10.1038/s41467-019-12013-y. PubMed
Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Yu D, Lee H, Hong J, Jung H, Jo Y, Oh BH, Park BO, Heo WD. Nat Methods. 2019 Nov;16(11):1095-1100. doi: 10.1038/s41592-019-0592-7. Epub 2019 Oct 14. PubMed
A curative combination cancer therapy achieves high fractional cell killing through low cross-resistance and drug additivity. Palmer AC, Chidley C, Sorger PK. Elife. 2019 Nov 19;8. pii: 50036. doi: 10.7554/eLife.50036. PubMed
AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage. Song G, Zhang F, Zhang X, Gao X, Zhu X, Fan D, Tian Y. Cell Rep. 2019 Nov 26;29(9):2579-2589.e4. doi: 10.1016/j.celrep.2019.10.078. PubMed
Robustness of Catalytically Dead Cas9 Activators in Human Pluripotent and Mesenchymal Stem Cells. Petazzi P, Torres-Ruiz R, Fidanza A, Roca-Ho H, Gutierrez-Aguera F, Castano J, Rodriguez-Perales S, Diaz de la Guardia R, Lopez-Millan B, Bigas A, Forrester LM, Bueno C, Menendez P. Mol Ther Nucleic Acids. 2020 Jun 5;20:196-204. doi: 10.1016/j.omtn.2020.02.009. Epub 2020 Feb 27. PubMed
CRISPR-Sunspot: Imaging of endogenous low-abundance RNA at the single-molecule level in live cells. Sun NH, Chen DY, Ye LP, Sheng G, Gong JJ, Chen BH, Lu YM, Han F. Theranostics. 2020 Sep 2;10(24):10993-11012. doi: 10.7150/thno.43094. eCollection 2020. PubMed
Transcription factor competition at the gamma-globin promoters controls hemoglobin switching. Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu JY, Sakon P, Pinello L, Yuan GC, Bauer DE, Orkin SH. Nat Genet. 2021 Apr;53(4):511-520. doi: 10.1038/s41588-021-00798-y. Epub 2021 Mar 1. PubMed
MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, Zhao S, Feng J, Huang G, Wan QL, Liu J, Huang H, Luan B, Wang D, Zhao X, Lin Y, Zhou Q, Zhang ZN, Rong Z. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174. PubMed

Associated Plasmids
Measurement of the nuclear concentration of alpha-ketoglutarate during adipocyte differentiation by using a fluorescence resonance energy transfer-based biosensor with nuclear localization signals. Suzuki T, Hayashi M, Komatsu T, Tanioka A, Nagasawa M, Tanimura-Inagaki K, Rahman MS, Masuda S, Yusa K, Sakai J, Shibata H, Inagaki T. Endocr J. 2021 Jul 15. doi: 10.1507/endocrj.EJ21-0255. PubMed
CRISPRi/a Screening with Human iPSCs. Nishiga M, Qi LS, Wu JC. Methods Mol Biol. 2021;2320:261-281. doi: 10.1007/978-1-0716-1484-6_23. PubMed
Age-related demethylation of the TDP-43 autoregulatory region in the human motor cortex. Koike Y, Sugai A, Hara N, Ito J, Yokoseki A, Ishihara T, Yamagishi T, Tsuboguchi S, Tada M, Ikeuchi T, Kakita A, Onodera O. Commun Biol. 2021 Sep 21;4(1):1107. doi: 10.1038/s42003-021-02621-0. PubMed
Locus-Specific DNA Methylation Editing in Melanoma Cell Lines Using a CRISPR-Based System. Smith J, Banerjee R, Waly R, Urbano A, Gimenez G, Day R, Eccles MR, Weeks RJ, Chatterjee A. Cancers (Basel). 2021 Oct 29;13(21). pii: cancers13215433. doi: 10.3390/cancers13215433. PubMed
Unexpected gene activation following CRISPR-Cas9-mediated genome editing. Manjon AG, Linder S, Teunissen H, Friskes A, Zwart W, de Wit E, Medema RH. EMBO Rep. 2022 Feb 3;23(2):e53902. doi: 10.15252/embr.202153902. Epub 2021 Dec 20. PubMed
A split prime editor with untethered reverse transcriptase and circular RNA template. Liu B, Dong X, Cheng H, Zheng C, Chen Z, Rodriguez TC, Liang SQ, Xue W, Sontheimer EJ. Nat Biotechnol. 2022 Apr 4. pii: 10.1038/s41587-022-01255-9. doi: 10.1038/s41587-022-01255-9. PubMed

Associated Plasmids
Secretory defects in pediatric osteosarcoma result from downregulation of selective COPII coatomer proteins. Wood RK, Flory AR, Mann MJ, Talbot LJ, Hendershot LM. iScience. 2022 Mar 17;25(4):104100. doi: 10.1016/j.isci.2022.104100. eCollection 2022 Apr 15. PubMed
Sequential enhancer state remodelling defines human germline competence and specification. Tang WWC, Castillo-Venzor A, Gruhn WH, Kobayashi T, Penfold CA, Morgan MD, Sun D, Irie N, Surani MA. Nat Cell Biol. 2022 Apr;24(4):448-460. doi: 10.1038/s41556-022-00878-z. Epub 2022 Apr 11. PubMed

Associated Plasmids
Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor. Danziger O, Patel RS, DeGrace EJ, Rosen MR, Rosenberg BR. PLoS Pathog. 2022 Apr 14;18(4):e1010464. doi: 10.1371/journal.ppat.1010464. eCollection 2022 Apr. PubMed

Associated Plasmids
Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions. Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton IB. Nucleic Acids Res. 2022 Aug 12;50(14):7842-7855. doi: 10.1093/nar/gkac582. PubMed
Activation of stably silenced genes by recruitment of a synthetic de-methylating module. Chan WF, Coughlan HD, Chen Y, Keenan CR, Smyth GK, Perkins AC, Johanson TM, Allan RS. Nat Commun. 2022 Sep 23;13(1):5582. doi: 10.1038/s41467-022-33181-4. PubMed

Associated Plasmids
CRISPR Activation/Interference Screen to Identify Genetic Networks in HDAC-Inhibitor-Resistant Cells. Jung P, Schmalbrock L, Wirth M. Methods Mol Biol. 2023;2589:429-454. doi: 10.1007/978-1-0716-2788-4_28. PubMed
G-quadruplex-guided RNA engineering to modulate CRISPR-based genomic regulation. Liu X, Cui S, Qi Q, Lei H, Zhang Y, Shen W, Fu F, Tian T, Zhou X. Nucleic Acids Res. 2022 Oct 28;50(19):11387-11400. doi: 10.1093/nar/gkac870. PubMed
Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential. Kneppers J, Severson TM, Siefert JC, Schol P, Joosten SEP, Yu IPL, Huang CF, Morova T, Altintas UB, Giambartolomei C, Seo JH, Baca SC, Carneiro I, Emberly E, Pasaniuc B, Jeronimo C, Henrique R, Freedman ML, Wessels LFA, Lack NA, Bergman AM, Zwart W. Nat Commun. 2022 Nov 30;13(1):7367. doi: 10.1038/s41467-022-35135-2. PubMed
IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia. Liu X, Sato N, Yabushita T, Li J, Jia Y, Tamura M, Asada S, Fujino T, Fukushima T, Yonezawa T, Tanaka Y, Fukuyama T, Tsuchiya A, Shikata S, Iwamura H, Kinouchi C, Komatsu K, Yamasaki S, Shibata T, Sasaki AT, Schibler J, Wunderlich M, O'Brien E, Mizukawa B, Mulloy JC, Sugiura Y, Takizawa H, Shibata T, Miyake K, Kitamura T, Goyama S. EMBO Mol Med. 2023 Jan 11;15(1):e15631. doi: 10.15252/emmm.202115631. Epub 2022 Dec 1. PubMed
Epigenome-wide association study identifies novel genes associated with ischemic stroke. Peng H, Palma-Gudiel H, Soriano-Tarraga C, Jimenez-Conde J, Zhang M, Zhang Y, Zhao J. Clin Epigenetics. 2023 Jun 27;15(1):106. doi: 10.1186/s13148-023-01520-x. PubMed
Multiplex single-cell chemical genomics reveals the kinase dependence of the response to targeted therapy. McFaline-Figueroa JL, Srivatsan S, Hill AJ, Gasperini M, Jackson DL, Saunders L, Domcke S, Regalado SG, Lazarchuck P, Alvarez S, Monnat RJ, Shendure J, Trapnell C. bioRxiv. 2023 Jun 14:2023.03.10.531983. doi: 10.1101/2023.03.10.531983. Preprint. PubMed
ASCT2 is the primary serine transporter in cancer cells. Conger KO, Chidley C, Ozgurses ME, Zhao H, Kim Y, Semina SE, Burns P, Rawat V, Sheldon R, Ben-Sahra I, Frasor J, Sorger PK, DeNicola GM, Coloff JL. bioRxiv [Preprint]. 2023 Oct 11:2023.10.09.561530. doi: 10.1101/2023.10.09.561530. PubMed

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