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pcDNA3-Tet2
(Plasmid #60939)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60939 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ten-Eleven Translocation 2
  • Alt name
    tet2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5763
  • Entrez Gene
    Tet2 (a.k.a. Ayu17-449, E130014J05Rik, mKIAA1546)
  • Promoter CMV
  • Tag / Fusion Protein
    • flag (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

*modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Tet2 was a gift from Yi Zhang (Addgene plasmid # 60939 ; http://n2t.net/addgene:60939 ; RRID:Addgene_60939)
  • For your References section:

    Regulation of TET protein stability by calpains. Wang Y, Zhang Y. Cell Rep. 2014 Jan 30;6(2):278-84. doi: 10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9. 10.1016/j.celrep.2013.12.031 PubMed 24412366