pU6-sgRNA EF1Alpha-puro-T2A-BFP Citations (53)
Originally described in: Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9. PubMed Journal
Articles Citing pU6-sgRNA EF1Alpha-puro-T2A-BFP
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| Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Radzisheuskaya A, Shlyueva D, Muller I, Helin K. Nucleic Acids Res. 2016 Oct 14;44(18):e141. Epub 2016 Jun 28. PubMed |
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.
Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS.
Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760.
PubMed
Associated Plasmids
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A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response.
Adamson B, Norman TM, Jost M, Cho MY, Nunez JK, Chen Y, Villalta JE, Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit A, Parnas O, Regev A, Weissman JS.
Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 10.1016/j.cell.2016.11.048.
PubMed
Associated Plasmids |
MELK is not necessary for the proliferation of basal-like breast cancer cells.
Huang HT, Seo HS, Zhang T, Wang Y, Jiang B, Li Q, Buckley DL, Nabet B, Roberts JM, Paulk J, Dastjerdi S, Winter GE, McLauchlan H, Moran J, Bradner JE, Eck MJ, Dhe-Paganon S, Zhao JJ, Gray NS.
Elife. 2017 Sep 19;6. pii: e26693. doi: 10.7554/eLife.26693.
PubMed
Associated Plasmids |
| Distinct roles for phosphoinositide 3-kinases gamma and delta in malignant B cell migration. Ali AY, Wu X, Eissa N, Hou S, Ghia JE, Murooka TT, Banerji V, Johnston JB, Lin F, Gibson SB, Marshall AJ. Leukemia. 2018 Sep;32(9):1958-1969. doi: 10.1038/s41375-018-0012-5. Epub 2018 Jan 31. PubMed |
| P53 and mTOR signalling determine fitness selection through cell competition during early mouse embryonic development. Bowling S, Di Gregorio A, Sancho M, Pozzi S, Aarts M, Signore M, D Schneider M, Martinez-Barbera JP, Gil J, Rodriguez TA. Nat Commun. 2018 May 2;9(1):1763. doi: 10.1038/s41467-018-04167-y. PubMed |
| The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins. Shurtleff MJ, Itzhak DN, Hussmann JA, Schirle Oakdale NT, Costa EA, Jonikas M, Weibezahn J, Popova KD, Jan CH, Sinitcyn P, Vembar SS, Hernandez H, Cox J, Burlingame AL, Brodsky JL, Frost A, Borner GH, Weissman JS. Elife. 2018 May 29;7. pii: 37018. doi: 10.7554/eLife.37018. PubMed |
| Specificity of RNAi, LNA and CRISPRi as loss-of-function methods in transcriptional analysis. Stojic L, Lun ATL, Mangei J, Mascalchi P, Quarantotti V, Barr AR, Bakal C, Marioni JC, Gergely F, Odom DT. Nucleic Acids Res. 2018 Jul 6;46(12):5950-5966. doi: 10.1093/nar/gky437. PubMed |
A Network of Noncoding Regulatory RNAs Acts in the Mammalian Brain.
Kleaveland B, Shi CY, Stefano J, Bartel DP.
Cell. 2018 Jul 12;174(2):350-362.e17. doi: 10.1016/j.cell.2018.05.022. Epub 2018 Jun 7.
PubMed
Associated Plasmids |
| Evidence for an Integrated Gene Repression Mechanism Based on mRNA Isoform Toggling in Human Cells. Hollerer I, Barker JC, Jorgensen V, Tresenrider A, Dugast-Darzacq C, Chan LY, Darzacq X, Tjian R, Unal E, Brar GA. G3 (Bethesda). 2019 Apr 9;9(4):1045-1053. doi: 10.1534/g3.118.200802. PubMed |
| Ceapins block the unfolded protein response sensor ATF6alpha by inducing a neomorphic inter-organelle tether. Torres SE, Gallagher CM, Plate L, Gupta M, Liem CR, Guo X, Tian R, Stroud RM, Kampmann M, Weissman JS, Walter P. Elife. 2019 May 31;8. pii: 46595. doi: 10.7554/eLife.46595. PubMed |
| Integrated analyses of murine breast cancer models reveal critical parallels with human disease. Rennhack JP, To B, Swiatnicki M, Dulak C, Ogrodzinski MP, Zhang Y, Li C, Bylett E, Ross C, Szczepanek K, Hanrahan W, Jayatissa M, Lunt SY, Hunter K, Andrechek ER. Nat Commun. 2019 Jul 22;10(1):3261. doi: 10.1038/s41467-019-11236-3. PubMed |
| PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD. Elguindy MM, Kopp F, Goodarzi M, Rehfeld F, Thomas A, Chang TC, Mendell JT. Elife. 2019 Jul 25;8. pii: 48625. doi: 10.7554/eLife.48625. PubMed |
| Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes. Temoche-Diaz MM, Shurtleff MJ, Nottingham RM, Yao J, Fadadu RP, Lambowitz AM, Schekman R. Elife. 2019 Aug 22;8. pii: 47544. doi: 10.7554/eLife.47544. PubMed |
| New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells. Bassaganyas L, Popa SJ, Horlbeck M, Puri C, Stewart SE, Campelo F, Ashok A, Butnaru CM, Brouwers N, Heydari K, Ripoche J, Weissman J, Rubinsztein DC, Schekman R, Malhotra V, Moreau K, Villeneuve J. J Cell Biol. 2019 Nov 4;218(11):3861-3879. doi: 10.1083/jcb.201902028. Epub 2019 Sep 5. PubMed |
| PRMT5 methylome profiling uncovers a direct link to splicing regulation in acute myeloid leukemia. Radzisheuskaya A, Shliaha PV, Grinev V, Lorenzini E, Kovalchuk S, Shlyueva D, Gorshkov V, Hendrickson RC, Jensen ON, Helin K. Nat Struct Mol Biol. 2019 Nov;26(11):999-1012. doi: 10.1038/s41594-019-0313-z. Epub 2019 Oct 14. PubMed |
| A curative combination cancer therapy achieves high fractional cell killing through low cross-resistance and drug additivity. Palmer AC, Chidley C, Sorger PK. Elife. 2019 Nov 19;8. pii: 50036. doi: 10.7554/eLife.50036. PubMed |
| A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division. Stojic L, Lun ATL, Mascalchi P, Ernst C, Redmond AM, Mangei J, Barr AR, Bousgouni V, Bakal C, Marioni JC, Odom DT, Gergely F. Nat Commun. 2020 Apr 15;11(1):1851. doi: 10.1038/s41467-020-14978-7. PubMed |
MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins through the Action of the ERH Protein.
Fang W, Bartel DP.
Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 10.1016/j.molcel.2020.01.026.
PubMed
Associated Plasmids |
| Targeted Perturb-seq enables genome-scale genetic screens in single cells. Schraivogel D, Gschwind AR, Milbank JH, Leonce DR, Jakob P, Mathur L, Korbel JO, Merten CA, Velten L, Steinmetz LM. Nat Methods. 2020 Jun;17(6):629-635. doi: 10.1038/s41592-020-0837-5. Epub 2020 Jun 1. PubMed |
| TETs compete with DNMT3 activity in pluripotent cells at thousands of methylated somatic enhancers. Charlton J, Jung EJ, Mattei AL, Bailly N, Liao J, Martin EJ, Giesselmann P, Brandl B, Stamenova EK, Muller FJ, Kiskinis E, Gnirke A, Smith ZD, Meissner A. Nat Genet. 2020 Aug;52(8):819-827. doi: 10.1038/s41588-020-0639-9. Epub 2020 Jun 8. PubMed |
| FOXO1 promotes HIV latency by suppressing ER stress in T cells. Vallejo-Gracia A, Chen IP, Perrone R, Besnard E, Boehm D, Battivelli E, Tezil T, Krey K, Raymond KA, Hull PA, Walter M, Habrylo I, Cruz A, Deeks S, Pillai S, Verdin E, Ott M. Nat Microbiol. 2020 Sep;5(9):1144-1157. doi: 10.1038/s41564-020-0742-9. Epub 2020 Jun 15. PubMed |
| Tuning the Expression of Long Noncoding RNA Loci with CRISPR Interference. Stojic L. Methods Mol Biol. 2020;2161:1-16. doi: 10.1007/978-1-0716-0680-3_1. PubMed |
| A Simple and Efficient Method to Generate Gene-Knockout and Transgenic Cell Lines. Liu J, Ge Y, Wang N, Wen J, Wang W, Zeng B, Chen GL. DNA Cell Biol. 2021 Feb;40(2):239-246. doi: 10.1089/dna.2020.6183. Epub 2020 Dec 31. PubMed |
| Proteome dynamics at broken replication forks reveal a distinct ATM-directed repair response suppressing DNA double-strand break ubiquitination. Nakamura K, Kustatscher G, Alabert C, Hodl M, Forne I, Volker-Albert M, Satpathy S, Beyer TE, Mailand N, Choudhary C, Imhof A, Rappsilber J, Groth A. Mol Cell. 2021 Mar 4;81(5):1084-1099.e6. doi: 10.1016/j.molcel.2020.12.025. Epub 2021 Jan 14. PubMed |
| Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis. Radzisheuskaya A, Shliaha PV, Grinev VV, Shlyueva D, Damhofer H, Koche R, Gorshkov V, Kovalchuk S, Zhan Y, Rodriguez KL, Johnstone AL, Keogh MC, Hendrickson RC, Jensen ON, Helin K. Mol Cell. 2021 Apr 15;81(8):1749-1765.e8. doi: 10.1016/j.molcel.2021.02.012. Epub 2021 Mar 2. PubMed |
| STING enhances cell death through regulation of reactive oxygen species and DNA damage. Hayman TJ, Baro M, MacNeil T, Phoomak C, Aung TN, Cui W, Leach K, Iyer R, Challa S, Sandoval-Schaefer T, Burtness BA, Rimm DL, Contessa JN. Nat Commun. 2021 Apr 19;12(1):2327. doi: 10.1038/s41467-021-22572-8. PubMed |
| Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance. Dahabieh MS, Huang F, Goncalves C, Flores Gonzalez RE, Prabhu S, Bolt A, Di Pietro E, Khoury E, Heath J, Xu ZY, Remy-Sarrazin J, Mann KK, Orthwein A, Boisvert FM, Braverman N, Miller WH, Del Rincon SV. Autophagy. 2021 Jun 21:1-19. doi: 10.1080/15548627.2021.1936932. PubMed |
| NORAD-induced Pumilio phase separation is required for genome stability. Elguindy MM, Mendell JT. Nature. 2021 Jul;595(7866):303-308. doi: 10.1038/s41586-021-03633-w. Epub 2021 Jun 9. PubMed |
| CRISPR Interference (CRISPRi) and CRISPR Activation (CRISPRa) to Explore the Oncogenic lncRNA Network. Morelli E, Gulla' A, Amodio N, Taiana E, Neri A, Fulciniti M, Munshi NC. Methods Mol Biol. 2021;2348:189-204. doi: 10.1007/978-1-0716-1581-2_13. PubMed |
| CRISPRi/a Screening with Human iPSCs. Nishiga M, Qi LS, Wu JC. Methods Mol Biol. 2021;2320:261-281. doi: 10.1007/978-1-0716-1484-6_23. PubMed |
| Extracellular vesicles from neurons promote neural induction of stem cells through cyclin D1. Song L, Tian X, Schekman R. J Cell Biol. 2021 Sep 6;220(9). pii: 212508. doi: 10.1083/jcb.202101075. Epub 2021 Jul 26. PubMed |
| Deficiency of the Lysosomal Protein CLN5 Alters Lysosomal Function and Movement. Basak I, Hansen RA, Ward ME, Hughes SM. Biomolecules. 2021 Sep 27;11(10). pii: biom11101412. doi: 10.3390/biom11101412. PubMed |
| Screening of ETO2-GLIS2-induced Super Enhancers identifies targetable cooperative dependencies in acute megakaryoblastic leukemia. Benbarche S, Lopez CK, Salataj E, Aid Z, Thirant C, Laiguillon MC, Lecourt S, Belloucif Y, Vaganay C, Antonini M, Hu J, da Silva Babinet A, Ndiaye-Lobry D, Pardieu B, Petit A, Puissant A, Chaumeil J, Mercher T, Lobry C. Sci Adv. 2022 Feb 11;8(6):eabg9455. doi: 10.1126/sciadv.abg9455. Epub 2022 Feb 9. PubMed |
| TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A. Brown AL, Wilkins OG, Keuss MJ, Hill SE, Zanovello M, Lee WC, Bampton A, Lee FCY, Masino L, Qi YA, Bryce-Smith S, Gatt A, Hallegger M, Fagegaltier D, Phatnani H, Newcombe J, Gustavsson EK, Seddighi S, Reyes JF, Coon SL, Ramos D, Schiavo G, Fisher EMC, Raj T, Secrier M, Lashley T, Ule J, Buratti E, Humphrey J, Ward ME, Fratta P. Nature. 2022 Mar;603(7899):131-137. doi: 10.1038/s41586-022-04436-3. Epub 2022 Feb 23. PubMed |
| Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens. Genolet O, Ravid Lustig L, Schulz EG. Methods Mol Biol. 2022;2520:1-24. doi: 10.1007/7651_2021_457. PubMed |
| The interplay between translational efficiency, poly(A) tails, microRNAs, and neuronal activation. Eisen TJ, Li JJ, Bartel DP. RNA. 2022 Jun;28(6):808-831. doi: 10.1261/rna.079046.121. Epub 2022 Mar 10. PubMed |
| A Genome-Wide Screen Identifies PDPK1 as a Target to Enhance the Efficacy of MEK1/2 Inhibitors in NRAS Mutant Melanoma. Cai W, Nguyen MQ, Wilski NA, Purwin TJ, Vernon M, Tiago M, Aplin AE. Cancer Res. 2022 Jul 18;82(14):2625-2639. doi: 10.1158/0008-5472.CAN-21-3217. PubMed |
| T cell stimulation remodels the latently HIV-1 infected cell population by differential activation of proviral chromatin. Lindqvist B, Jutte BB, Love L, Assi W, Roux J, Sonnerborg A, Tezil T, Verdin E, Svensson JP. PLoS Pathog. 2022 Jun 6;18(6):e1010555. doi: 10.1371/journal.ppat.1010555. eCollection 2022 Jun. PubMed |
| Small-Molecule-Mediated Split-Aptamer Assembly for Inducible CRISPR-dCas9 Transcription Activation. Liu XH, Li BR, Ying ZM, Tang LJ, Wang F, Jiang JH. ACS Chem Biol. 2022 Jul 15;17(7):1769-1777. doi: 10.1021/acschembio.2c00101. Epub 2022 Jun 14. PubMed |
| Building regulatory landscapes reveals that an enhancer can recruit cohesin to create contact domains, engage CTCF sites and activate distant genes. Rinzema NJ, Sofiadis K, Tjalsma SJD, Verstegen MJAM, Oz Y, Valdes-Quezada C, Felder AK, Filipovska T, van der Elst S, de Andrade Dos Ramos Z, Han R, Krijger PHL, de Laat W. Nat Struct Mol Biol. 2022 Jun;29(6):563-574. doi: 10.1038/s41594-022-00787-7. Epub 2022 Jun 16. PubMed |
| CRISPR Activation/Interference Screen to Identify Genetic Networks in HDAC-Inhibitor-Resistant Cells. Jung P, Schmalbrock L, Wirth M. Methods Mol Biol. 2023;2589:429-454. doi: 10.1007/978-1-0716-2788-4_28. PubMed |
| Inhibition of the MNK1/2-eIF4E Axis Augments Palbociclib-Mediated Antitumor Activity in Melanoma and Breast Cancer. Prabhu SA, Moussa O, Goncalves C, LaPierre JH, Chou H, Huang F, Richard VR, Ferruzo PYM, Guettler EM, Soria-Bretones I, Kirby L, Gagnon N, Su J, Silvester J, Krisna SS, Rose AAN, Sheppard KE, Cescon DW, Mallette FA, Zahedi RP, Borchers CH, Del Rincon SV, Miller WH. Mol Cancer Ther. 2023 Feb 1;22(2):192-204. doi: 10.1158/1535-7163.MCT-22-0092. PubMed |
| Production of CRISPRi-engineered primary human mammary epithelial cells with baboon envelope pseudotyped lentiviral vectors. Pastor S, Wicinski J, Charafe-Jauffret E, Verhoeyen E, Guittard G, Ginestier C. STAR Protoc. 2023 Jan 19;4(1):102055. doi: 10.1016/j.xpro.2023.102055. PubMed |
| Histone malonylation is regulated by SIRT5 and KAT2A. Zhang R, Bons J, Scheidemantle G, Liu X, Bielska O, Carrico C, Rose J, Heckenbach I, Scheibye-Knudsen M, Schilling B, Verdin E. iScience. 2023 Feb 13;26(3):106193. doi: 10.1016/j.isci.2023.106193. eCollection 2023 Mar 17. PubMed |
| Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein. Zhang C, Schekman R. Elife. 2023 Mar 10;12:e84391. doi: 10.7554/eLife.84391. PubMed |
| Identification of chemotherapy targets reveals a nucleus-to-mitochondria ROS sensing pathway. Zhang J, Simpson CM, Berner J, Chong HB, Fang J, Sahin ZO, Weiss-Sadan T, Possemato AP, Harry S, Takahashi M, Yang TY, Richter M, Patel H, Smith AE, Carlin AD, Hubertus de Groot AF, Wolf K, Shi L, Wei TY, Durr BR, Chen NJ, Vornbaumen T, Wichmann NO, Pooladanda V, Matoba Y, Kumar S, Kim E, Bouberhan S, Olivia E, Rueda B, Bardeesy N, Liau B, Lawrence M, Stokes MP, Beausoleil SA, Bar-Peled L. bioRxiv. 2023 Mar 11:2023.03.11.532189. doi: 10.1101/2023.03.11.532189. Preprint. PubMed |
| CRISPR interference to evaluate modifiers of C9ORF72-mediated toxicity in FTD. Pickles S, Zanetti Alepuz D, Koike Y, Yue M, Tong J, Liu P, Zhou Y, Jansen-West K, Daughrity LM, Song Y, DeTure M, Oskarsson B, Graff-Radford NR, Boeve BF, Petersen RC, Josephs KA, Dickson DW, Ward ME, Dong L, Prudencio M, Cook CN, Petrucelli L. Front Cell Dev Biol. 2023 Aug 7;11:1251551. doi: 10.3389/fcell.2023.1251551. eCollection 2023. PubMed |
| Mapping the Genetic Interaction Network of PARP inhibitor Response. Simpson D, Ling J, Jing Y, Adamson B. bioRxiv [Preprint]. 2023 Aug 20:2023.08.19.553986. doi: 10.1101/2023.08.19.553986. PubMed |
| Comprehensive synergy mapping links a BAF- and NSL-containing "supercomplex" to the transcriptional silencing of HIV-1. Li Z, Deeks SG, Ott M, Greene WC. Cell Rep. 2023 Sep 26;42(9):113055. doi: 10.1016/j.celrep.2023.113055. Epub 2023 Sep 7. PubMed |
| ORAOV1, CCND1, and MIR548K Are the Driver Oncogenes of the 11q13 Amplicon in Squamous Cell Carcinoma. Mahieu CI, Mancini AG, Vikram EP, Planells-Palop V, Joseph NM, Tward AD. Mol Cancer Res. 2024 Feb 1;22(2):152-168. doi: 10.1158/1541-7786.MCR-23-0746. PubMed |
| GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers. Ravid Lustig L, Sampath Kumar A, Schwammle T, Dunkel I, Noviello G, Limberg E, Weigert R, Pacini G, Buschow R, Ghauri A, Stotzel M, Wittler L, Meissner A, Schulz EG. Nat Cell Biol. 2023 Nov;25(11):1704-1715. doi: 10.1038/s41556-023-01266-x. Epub 2023 Nov 6. PubMed |
| Selective gene expression maintains human tRNA anticodon pools during differentiation. Gao L, Behrens A, Rodschinka G, Forcelloni S, Wani S, Strasser K, Nedialkova DD. Nat Cell Biol. 2024 Jan;26(1):100-112. doi: 10.1038/s41556-023-01317-3. Epub 2024 Jan 8. PubMed |
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