Purposefor generation of a eGFP specific sgRNA from a T7 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61051||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerJoung Lab, Addgene plasmid # 42250
Growth in Bacteria
Gene/Insert nameEGFP sgRNA
- Promoter T7
- Cloning method Unknown
- 5′ sequencing primer M13 (Common Sequencing Primers)
eGFP sgRNA = GGCGAGGGCGATGCCACCTA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:DR274-eGFP sgRNA was a gift from Filippo Del Bene (Addgene plasmid # 61051 ; http://n2t.net/addgene:61051 ; RRID:Addgene_61051)
For your References section:Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F. Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 2013 Oct 31. 10.1101/gr.161638.113 PubMed 24179142