pHIP_RNA2
(Plasmid
#61260)
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PurposeOrsay virus RNA2 genome segment expression plasmid for virus reverse genetics
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepPD49.83
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Backbone manufacturerFire Lab Vector Kit
- Backbone size w/o insert (bp) 3826
- Total vector size (bp) 6435
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Modifications to backboneHDV ribozyme added
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOrsay RNA2
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SpeciesNematode virus
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Insert Size (bp)2574
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GenBank IDHM030971.2
- Promoter heat shock promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Pst1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer CGG AAC GTT GAG CTG GAC GGA
- 3′ sequencing primer TTG GACTTA GAAGTCAGA GGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHIP_RNA2 was a gift from David Wang (Addgene plasmid # 61260 ; http://n2t.net/addgene:61260 ; RRID:Addgene_61260) -
For your References section:
Engineering Recombinant Orsay Virus Directly in the Metazoan Host Caenorhabditis elegans. Jiang H, Franz CJ, Wang D. J Virol. 2014 Oct 15;88(20):11774-81. doi: 10.1128/JVI.01630-14. Epub 2014 Jul 30. 10.1128/JVI.01630-14 PubMed 25078701
