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pZDonor-Nm-Cas9-gRNA-hU6
(Plasmid #61366)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61366 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pZdonor
  • Backbone manufacturer
    Sigma-Aldrich
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Promoter human U6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZDonor-Nm-Cas9-gRNA-hU6 was a gift from Charles Gersbach (Addgene plasmid # 61366)
  • For your References section:

    Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. 10.1038/nbt.3199 PubMed 25849900