pAD-g6
(Plasmid #61497)

• Purpose: For expression of C. briggsae gld-1, used for rescue of C. elegans q485 homozygotes
• Depositing Lab: Eric Haag
• Publication: Beadell et al Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19672-7. doi: 10.1073/pnas.1108068108. Epub 2011 Nov 21.

Backbone

• Vector backbone: pCR50
• Backbone manufacturer: a gift of C. Richie and A. Golden, National Institutes of Health
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gld-1
• Alt name: Cbr-gld-1
• Species: Caenorhabditis briggsae
• Entrez Gene: Cbr-gld-1 (a.k.a. CBG00303)
• Promoter: gld1
• Tags / Fusion Proteins:
  • His (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: BamHI (destroyed during cloning)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert contains 923 nt upstream of start codon and 1,354 nt downstream of stop codon.