pET15b-Opt13
(Plasmid
#61698)
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PurposeExpresses PTDH mutant Opt13
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61698 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET15b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOpt13
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SpeciesPseudomonas stutzeri
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Insert Size (bp)1014
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pBRrevBam; T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b-Opt13 was a gift from Huimin Zhao (Addgene plasmid # 61698 ; http://n2t.net/addgene:61698 ; RRID:Addgene_61698) -
For your References section:
Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis. McLachlan MJ, Johannes TW, Zhao H. Biotechnol Bioeng. 2008 Feb 1;99(2):268-74. 10.1002/bit.21546 PubMed 17615560