PurposeLuciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61796||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6273
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemouse Ywhab 3'UTR with miR-200c MRE-1 mutated
SpeciesM. musculus (mouse)
Insert Size (bp)1862
MutationmiR-200c MRE-1 mutated
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer 5'-GTCCGCAACTACAACGCCTACCTT-3' (Common Sequencing Primers)
Terms and Licenses
Note that the UTR sequence in this plasmid may have some minor discrepancies with top GenBank hits
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT was a gift from Judy Lieberman (Addgene plasmid # 61796 ; http://n2t.net/addgene:61796 ; RRID:Addgene_61796)
For your References section:miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Perdigao-Henriques R, Petrocca F, Altschuler G, Thomas MP, Le MT, Tan SM, Hide W, Lieberman J. Oncogene. 2015 Mar 23. doi: 10.1038/onc.2015.69. 10.1038/onc.2015.69 PubMed 25798844