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pFA6a URA3
(Plasmid #61924)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61924 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Total vector size (bp) 3628
  • Vector type
    Yeast genomic targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    URA3
  • Species
    S. cerevisiae (budding yeast)
  • GenBank ID
    AB540657.1

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The amplified region is functional, but does not overlap with the URA3 deletion region in BY4741 and derivatives, so will not recombine at the endogenous locus in this background.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a URA3 was a gift from Jon Houseley & David Tollervey (Addgene plasmid # 61924 ; http://n2t.net/addgene:61924 ; RRID:Addgene_61924)
  • For your References section:

    Repeat expansion in the budding yeast ribosomal DNA can occur independently of the canonical homologous recombination machinery. Houseley J, Tollervey D. Nucleic Acids Res. 2011 Nov 1;39(20):8778-91. doi: 10.1093/nar/gkr589. Epub 2011 Jul 17. 10.1093/nar/gkr589 PubMed 21768125