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3xFLAG-dCas9/pTEF1p-CYC1t
(Plasmid #62190)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62190 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p414
  • Backbone size w/o insert (bp) 5385
  • Total vector size (bp) 9596
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    3xFLAG-dCas9
  • Species
    Synthetic; S. pyogenes
  • Insert Size (bp)
    4212
  • Mutation
    human codon-optimized, D10A, H840A
  • Promoter TEF1 promoter
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    George Church (Addgene plasmid 43802)
  • Terms and Licenses

Depositor Comments

This plasmid is compatible with gRNA expression plasmids for budding yeast, such as Addgene Plasmid #43803 ( http://www.addgene.org/43803 ).

For more information on Fujii Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/fujii/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFLAG-dCas9/pTEF1p-CYC1t was a gift from Hodaka Fujii (Addgene plasmid # 62190)
  • For your References section:

    An enChIP system for the analysis of bacterial genome functions. Fujita T, Yuno M, Fujii H. BMC Res Notes. 2018 Jun 14;11(1):387. doi: 10.1186/s13104-018-3486-3. 10.1186/s13104-018-3486-3 PubMed 29898790