pLenti SpBsmBI sgRNA Hygro Citations (27)
Originally described in: Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells.Pham H, Kearns NA, Maehr R Methods Mol Biol. 2016;1358:43-57. doi: 10.1007/978-1-4939-3067-8_3. PubMed Journal
Articles Citing pLenti SpBsmBI sgRNA Hygro
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| Controlling transcription in human pluripotent stem cells using CRISPR-effectors. Genga RM, Kearns NA, Maehr R. Methods. 2015 Oct 23. pii: S1046-2023(15)30132-8. doi: 10.1016/j.ymeth.2015.10.014. PubMed |
| A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures. Wang G, Zhao N, Berkhout B, Das AT. Cell Rep. 2016 Dec 13;17(11):2819-2826. doi: 10.1016/j.celrep.2016.11.057. PubMed |
| CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors. Ma Y, Walsh MJ, Bernhardt K, Ashbaugh CW, Trudeau SJ, Ashbaugh IY, Jiang S, Jiang C, Zhao B, Root DE, Doench JG, Gewurz BE. Cell Host Microbe. 2017 May 10;21(5):580-591.e7. doi: 10.1016/j.chom.2017.04.005. PubMed |
| Combinatorial CRISPR-Cas9 and RNA Interference Attack on HIV-1 DNA and RNA Can Lead to Cross-Resistance. Zhao N, Wang G, Das AT, Berkhout B. Antimicrob Agents Chemother. 2017 Nov 22;61(12). pii: AAC.01486-17. doi: 10.1128/AAC.01486-17. Print 2017 Dec. PubMed |
| Dynamic evolution of regulatory element ensembles in primate CD4(+) T cells. Danko CG, Choate LA, Marks BA, Rice EJ, Wang Z, Chu T, Martins AL, Dukler N, Coonrod SA, Tait Wojno ED, Lis JT, Kraus WL, Siepel A. Nat Ecol Evol. 2018 Mar;2(3):537-548. doi: 10.1038/s41559-017-0447-5. Epub 2018 Jan 29. PubMed |
| Co-optation of Tandem DNA Repeats for the Maintenance of Mesenchymal Identity. Balestrieri C, Alfarano G, Milan M, Tosi V, Prosperini E, Nicoli P, Palamidessi A, Scita G, Diaferia GR, Natoli G. Cell. 2018 May 17;173(5):1150-1164.e14. doi: 10.1016/j.cell.2018.03.081. Epub 2018 Apr 26. PubMed |
CK1alpha and IRF4 are essential and independent effectors of immunomodulatory drugs in primary effusion lymphoma.
Patil A, Manzano M, Gottwein E.
Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418. Epub 2018 Jun 28.
PubMed
Associated Plasmids |
| The Impact of HIV-1 Genetic Diversity on CRISPR-Cas9 Antiviral Activity and Viral Escape. Darcis G, Binda CS, Klaver B, Herrera-Carrillo E, Berkhout B, Das AT. Viruses. 2019 Mar 13;11(3). pii: v11030255. doi: 10.3390/v11030255. PubMed |
| Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs. Pontis J, Planet E, Offner S, Turelli P, Duc J, Coudray A, Theunissen TW, Jaenisch R, Trono D. Cell Stem Cell. 2019 May 2;24(5):724-735.e5. doi: 10.1016/j.stem.2019.03.012. Epub 2019 Apr 18. PubMed |
| CRISPR/Cas9 Screens Reveal Multiple Layers of B cell CD40 Regulation. Jiang C, Trudeau SJ, Cheong TC, Guo R, Teng M, Wang LW, Wang Z, Pighi C, Gautier-Courteille C, Ma Y, Jiang S, Wang C, Zhao B, Paillard L, Doench JG, Chiarle R, Gewurz BE. Cell Rep. 2019 Jul 30;28(5):1307-1322.e8. doi: 10.1016/j.celrep.2019.06.079. PubMed |
| Re-programing Chromatin with a Bifunctional LSD1/HDAC Inhibitor Induces Therapeutic Differentiation in DIPG. Anastas JN, Zee BM, Kalin JH, Kim M, Guo R, Alexandrescu S, Blanco MA, Giera S, Gillespie SM, Das J, Wu M, Nocco S, Bonal DM, Nguyen QD, Suva ML, Bernstein BE, Alani R, Golub TR, Cole PA, Filbin MG, Shi Y. Cancer Cell. 2019 Nov 11;36(5):528-544.e10. doi: 10.1016/j.ccell.2019.09.005. Epub 2019 Oct 17. PubMed |
| CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA. Binda CS, Klaver B, Berkhout B, Das AT. Viruses. 2020 Mar 18;12(3). pii: v12030330. doi: 10.3390/v12030330. PubMed |
| DNA methylation enzymes and PRC1 restrict B-cell Epstein-Barr virus oncoprotein expression. Guo R, Zhang Y, Teng M, Jiang C, Schineller M, Zhao B, Doench JG, O'Reilly RJ, Cesarman E, Giulino-Roth L, Gewurz BE. Nat Microbiol. 2020 Aug;5(8):1051-1063. doi: 10.1038/s41564-020-0724-y. Epub 2020 May 18. PubMed |
| Epstein-Barr Virus Induced Cytidine Metabolism Roles in Transformed B-Cell Growth and Survival. Liang JH, Wang C, Yiu SPT, Zhao B, Guo R, Gewurz BE. mBio. 2021 Aug 31;12(4):e0153021. doi: 10.1128/mBio.01530-21. Epub 2021 Jul 20. PubMed |
| Multiple stages of evolutionary change in anthrax toxin receptor expression in humans. Choate LA, Barshad G, McMahon PW, Said I, Rice EJ, Munn PR, Lewis JJ, Danko CG. Nat Commun. 2021 Nov 15;12(1):6590. doi: 10.1038/s41467-021-26854-z. PubMed |
| Four-dimensional nuclear speckle phase separation dynamics regulate proteostasis. Dion W, Ballance H, Lee J, Pan Y, Irfan S, Edwards C, Sun M, Zhang J, Zhang X, Liu S, Zhu B. Sci Adv. 2022 Jan 7;8(1):eabl4150. doi: 10.1126/sciadv.abl4150. Epub 2022 Jan 5. PubMed |
| Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments. Yiu SPT, Guo R, Zerbe C, Weekes MP, Gewurz BE. Cell Rep. 2022 Mar 8;38(10):110411. doi: 10.1016/j.celrep.2022.110411. PubMed |
| A combinatorial CRISPR-Cas12a attack on HIV DNA. Fan M, Berkhout B, Herrera-Carrillo E. Mol Ther Methods Clin Dev. 2022 Feb 26;25:43-51. doi: 10.1016/j.omtm.2022.02.010. eCollection 2022 Jun 9. PubMed |
| Developing CRISPR/Cas9-Mediated Fluorescent Reporter Human Pluripotent Stem-Cell Lines for High-Content Screening. Vojnits K, Nakanishi M, Porras D, Kim Y, Feng Z, Golubeva D, Bhatia M. Molecules. 2022 Apr 9;27(8). pii: molecules27082434. doi: 10.3390/molecules27082434. PubMed |
| Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis. Simo-Riudalbas L, Offner S, Planet E, Duc J, Abrami L, Dind S, Coudray A, Coto-Llerena M, Ercan C, Piscuoglio S, Andersen CL, Bramsen JB, Trono D. Nat Commun. 2022 Aug 20;13(1):4913. doi: 10.1038/s41467-022-32649-7. PubMed |
| Workflow for Performing Genetic Manipulation in Human Trophoblast Stem Cells Using CRISPR/Cas9 Technology. Wu H, Wang Y, Wang H. Methods Mol Biol. 2022 Dec 15. doi: 10.1007/7651_2022_464. PubMed |
| GDNF-RET signaling and EGR1 form a positive feedback loop that promotes tamoxifen resistance via cyclin D1. Marks BA, Pipia IM, Mukai C, Horibata S, Rice EJ, Danko CG, Coonrod SA. BMC Cancer. 2023 Feb 10;23(1):138. doi: 10.1186/s12885-023-10559-1. PubMed |
| CRISPR screens identify novel regulators of cFLIP dependency and ligand-independent, TRAIL-R1-mediated cell death. Kuehnle N, Osborne SM, Liang Z, Manzano M, Gottwein E. Cell Death Differ. 2023 May;30(5):1221-1234. doi: 10.1038/s41418-023-01133-0. Epub 2023 Feb 18. PubMed |
| Characterization of Target Gene Regulation by the Two Epstein-Barr Virus Oncogene LMP1 Domains Essential for B-cell Transformation. Mitra B, Guo R, Burton EM, Murray-Nerger LA, Gewurz BE. bioRxiv. 2023 Apr 10:2023.04.10.536234. doi: 10.1101/2023.04.10.536234. Preprint. PubMed |
| An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation. Yiu SPT, Zerbe C, Vanderwall D, Huttlin EL, Weekes MP, Gewurz BE. Mol Cell. 2023 Jul 6;83(13):2367-2386.e15. doi: 10.1016/j.molcel.2023.05.018. Epub 2023 Jun 12. PubMed |
| CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures. Fan M, Bao Y, Berkhout B, Herrera-Carrillo E. Biomed Pharmacother. 2023 Sep;165:115046. doi: 10.1016/j.biopha.2023.115046. Epub 2023 Jun 28. PubMed |
| Avian influenza A viruses exhibit plasticity in sialylglycoconjugate receptor usage in human lung cells. Liang C-Y, Huang I, Han J, Sownthirarajan B, Kulhankova K, Murray NB, Taherzadeh M, Archer-Hartmann S, Pepi L, Manivasagam S, Plung J, Sturtz M, Yu Y, Vogel OA, Kandasamy M, Gourronc FA, Klingelhutz AJ, Choudhury B, Rong L, Perez JT, Azadi P, McCray PB Jr, Neelamegham S, Manicassamy B. J Virol. 2023 Nov 30;97(11):e0090623. doi: 10.1128/jvi.00906-23. Epub 2023 Oct 16. PubMed |
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