pTargetF
(Plasmid #62226)

• Purpose: Constitutive expression of sgRNA without donor editing template DNA
• Depositing Lab: Sheng Yang
• Publication: Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.

Backbone

• Vector backbone: Trc99a
• Backbone size w/o insert (bp): 2000
• Modifications to backbone: Replaced Amp with aadA
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sgRNA
• gRNA/shRNA sequence: GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTG
• Species: Synthetic
• Promoter: pij23119

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: MoClo-F (agcgaggaagcggaagagcg)

Resource Information

• Supplemental Documents:
  • Genbank
• Articles Citing this Plasmid:
  • 56 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry