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RHD_exon1_TALEN_R
(Plasmid #62240)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62240 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 3739
  • Total vector size (bp) 6763
  • Modifications to backbone
    with a cmv promoter but without neo selection cassette
  • Vector type
    TALEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human RHD_exon1_TALEN_Right
  • Insert Size (bp)
    3024
  • Promoter CMV
  • Tag / Fusion Protein
    • HA tag, NLS (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid is purchased form Toolgen (Seoul, Korea).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RHD_exon1_TALEN_R was a gift from Hyongbum Kim (Addgene plasmid # 62240 ; http://n2t.net/addgene:62240 ; RRID:Addgene_62240)
  • For your References section:

    Rh D blood group conversion using transcription activator-like effector nucleases. Kim YH, Kim HO, Baek EJ, Kurita R, Cha HJ, Nakamura Y, Kim H. Nat Commun. 2015 Jun 16;6:7451. doi: 10.1038/ncomms8451. 10.1038/ncomms8451 PubMed 26078220