pDSARN
(Plasmid
#62295)
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Purpose(Empty Backbone) docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR P4r-P3r
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Backbone manufacturerInvitrogen
- Backbone size (bp) 5247
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Modifications to backboneLacZ cassette for blue-white selection flanked by BsaI sites for GoldenGate cloning of inserts. SV40 terminator. 3xP3-DsRed2nls marker to select transgenic insects.
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer M13F (after cloning! present twice before cloning)
- 3′ sequencing primer M13R (after cloning! present twice before cloning) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDSARN was a gift from Eric Marois (Addgene plasmid # 62295 ; http://n2t.net/addgene:62295 ; RRID:Addgene_62295) -
For your References section:
Tools for Anopheles gambiae Transgenesis. Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, Blandin SA, Marois E. G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 10.1534/g3.115.016808. 10.1534/g3.115.016808 PubMed 25869647
