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pBSKΔB-TRE-dCas9-EGFP
(Plasmid #62347)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62347 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    4995
  • Promoter TRE
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • SV40 NLS (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSKΔB-TRE-dCas9-EGFP was a gift from Hiroshi Ochiai (Addgene plasmid # 62347 ; http://n2t.net/addgene:62347 ; RRID:Addgene_62347)
  • For your References section:

    Simultaneous live imaging of the transcription and nuclear position of specific genes. Ochiai H, Sugawara T, Yamamoto T. Nucleic Acids Res. 2015 Jun 19. pii: gkv624. 10.1093/nar/gkv624 PubMed 26092696