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pJW1517
(Plasmid #62387)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62387 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a
  • Total vector size (bp) 7164
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OSM1-EGFP fusion
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2244
  • Mutation
    Methionine 33 mutated to Alanine
  • Promoter Adh1p
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW1517 was a gift from Jonathan Weissman (Addgene plasmid # 62387 ; http://n2t.net/addgene:62387 ; RRID:Addgene_62387)
  • For your References section:

    Targeting and plasticity of mitochondrial proteins revealed by proximity-specific ribosome profiling. Williams CC, Jan CH, Weissman JS. Science. 2014 Nov 7;346(6210):748-51. doi: 10.1126/science.1257522. 10.1126/science.1257522 PubMed 25378625