Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pKDsgRNA-p15 Citations (17)

Originally described in: The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.
Reisch CR, Prather KL Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
PubMed Journal

Articles Citing pKDsgRNA-p15

Articles
Scarless Cas9 Assisted Recombineering (no-SCAR) in Escherichia coli, an Easy-to-Use System for Genome Editing. Reisch CR, Prather KLJ. Curr Protoc Mol Biol. 2017 Jan 5;117:31.8.1-31.8.20. doi: 10.1002/cpmb.29. PubMed
vB_EcoS_IME347 a novel T1-like Escherichia coli bacteriophage. Li P, Lin H, Mi Z, Tong Y, Wang J. J Basic Microbiol. 2018 Nov;58(11):968-976. doi: 10.1002/jobm.201800271. Epub 2018 Aug 26. PubMed
Comparative genomics analysis of pTEM-2262, an MDR plasmid from Citrobacter freundii, harboring two unclassified replicons. Li M, Li F, Mi Z, Zhao Y, Zhang X, Jiang Z, Pei G, Zhou L, Tong Y, Zhao B. Future Microbiol. 2018 Nov;13:1657-1668. doi: 10.2217/fmb-2018-0243. Epub 2018 Nov 30. PubMed
Allosteric Feedback Inhibition Enables Robust Amino Acid Biosynthesis in E. coli by Enforcing Enzyme Overabundance. Sander T, Farke N, Diehl C, Kuntz M, Glatter T, Link H. Cell Syst. 2019 Jan 23;8(1):66-75.e8. doi: 10.1016/j.cels.2018.12.005. Epub 2019 Jan 9. PubMed
Escherichia coli vectors having stringently repressible replication origins allow a streamlining of Crispr/Cas9 gene editing. Srinivas S, Hu Z, Cronan JE. Plasmid. 2019 May;103:53-62. doi: 10.1016/j.plasmid.2019.04.004. Epub 2019 Apr 29. PubMed
Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis. Li P, Lin H, Mi Z, Xing S, Tong Y, Wang J. Front Microbiol. 2019 Apr 18;10:850. doi: 10.3389/fmicb.2019.00850. eCollection 2019. PubMed
A Combinatorial Approach to Optimize the Production of Curcuminoids From Tyrosine in Escherichia coli. Rodrigues JL, Gomes D, Rodrigues LR. Front Bioeng Biotechnol. 2020 Feb 7;8:59. doi: 10.3389/fbioe.2020.00059. eCollection 2020. PubMed
Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits. Contreras-Llano LE, Meyer C, Liu Y, Sarker M, Lim S, Longo ML, Tan C. Nat Commun. 2020 Jun 19;11(1):3138. doi: 10.1038/s41467-020-16900-7. PubMed
Sequence analysis and specificity of distinct types of menaquinone methyltransferases indicate the widespread potential of methylmenaquinone production in bacteria and archaea. Wilkens D, Meusinger R, Hein S, Simon J. Environ Microbiol. 2021 Mar;23(3):1407-1421. doi: 10.1111/1462-2920.15344. Epub 2020 Dec 15. PubMed
Metabolome and proteome analyses reveal transcriptional misregulation in glycolysis of engineered E. coli. Wang CY, Lempp M, Farke N, Donati S, Glatter T, Link H. Nat Commun. 2021 Aug 13;12(1):4929. doi: 10.1038/s41467-021-25142-0. PubMed
CRISPR/Cas9-mediated generation of auxotrophic Edwardsiella piscicida mutants and immunization in olive flounder (Paralichthys olivaceus). Lee EG, Kwak JS, Kim KH. Fish Shellfish Immunol. 2022 Mar;122:98-105. doi: 10.1016/j.fsi.2022.01.040. Epub 2022 Feb 1. PubMed
Comprehensive Mutational Analysis of the Lasso Peptide Klebsidin. Hills E, Woodward TJ, Fields S, Brandsen BM. ACS Chem Biol. 2022 Apr 15;17(4):998-1010. doi: 10.1021/acschembio.2c00148. Epub 2022 Mar 22. PubMed
Deletion of FRT-sites by no-SCAR recombineering in Escherichia coli. Rangarajan AA, Yilmaz C, Schnetz K. Microbiology (Reading). 2022 Apr;168(4). doi: 10.1099/mic.0.001173. PubMed

Associated Plasmids

Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System. Ellington AJ, Reisch CR. Methods Mol Biol. 2022;2479:119-133. doi: 10.1007/978-1-0716-2233-9_9. PubMed
DELETE_Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli. Ellington AJ, Reisch CR. Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 10.1093/synbio/ysac007. eCollection 2022. PubMed
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets. Chaves GL, Batista RS, Cunha JS, Oliveira DB, da Silva MR, Pisani GFD, Selistre-de-Araujo HS, Zangirolami TC, da Silva AJ. N Biotechnol. 2022 Dec 25;72:80-88. doi: 10.1016/j.nbt.2022.10.002. Epub 2022 Oct 19. PubMed
Generation of bright autobioluminescent bacteria by chromosomal integration of the improved lux operon ilux2. Gregor C. Sci Rep. 2022 Nov 9;12(1):19039. doi: 10.1038/s41598-022-22068-5. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.