pLPC-Flag-PML-IV
(Plasmid #62804)

• Purpose: expresses the isoform IV of PML (promyelocytic leukemia protein)
• Depositing Lab: Gerardo Ferbeyre
• Publication: Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi: 10.1101/gad.1975111.

Backbone

• Vector backbone: pLPC
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7480
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PML-IV
• Species: H. sapiens (human)
• Insert Size (bp): 1902
• Entrez Gene: PML (a.k.a. MYL, PP8675, RNF71, TRIM19)
• Tag / Fusion Protein:
  • 3X-FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: taggcgtgtacggtggga
• 3′ sequencing primer: acctgtaggtttggcaag

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The PML isoform in this plasmid is referred to as PML-IV according to the nomenclature established by Jensen, Shiels and Freemont Oncogene. 2001 Oct 29;20(49):7223-33. The GenBank nomenclature for PML-IV is PML isoform 6 (GenBank ID NM_002675.3).