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pMIR-Report-Luc-KLF4-K6S
(Plasmid #62983)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62983 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMIR-Report-LUC
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 7028
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KLF4 partial sequence
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    558
  • Mutation
    Fragment 2086-2639 of KLF4
  • GenBank ID
    AF105036
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Promoter CMV
  • Tag / Fusion Protein
    • firefly luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PmeI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer Luc-F
  • 3′ sequencing primer M13pUC-Fwd
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-Report-Luc-KLF4-K6S was a gift from Michael Ruppert (Addgene plasmid # 62983 ; http://n2t.net/addgene:62983 ; RRID:Addgene_62983)
  • For your References section:

    A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. 10.1128/MCB.01189-10 PubMed 21518959