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Or22a-Gal4
(Plasmid #63023)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 63023 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCasperAUG-Gal4-X
  • Backbone manufacturer
    Leslie Vosshall Lab
  • Backbone size w/o insert (bp) 12000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosophila melanogaster Or22a promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    7717
  • Tag / Fusion Protein
    • Drosophila ADH (aa's 1-18) fused to Yeast Gal4 (aa's 1-881) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asp718I (not destroyed)
  • 3′ cloning site Asp718I (not destroyed)
  • 5′ sequencing primer pCasper-F 5'-gggttttattaacttacat-3'
  • 3′ sequencing primer GAL4Nterm-R 5'-tcagatgtgccctagtcagc-3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Or22a-Gal4 was a gift from Leslie Vosshall (Addgene plasmid # 63023)
  • For your References section:

    Genetic and functional subdivision of the Drosophila antennal lobe. Fishilevich E, Vosshall LB. Curr Biol. 2005 Sep 6;15(17):1548-53. 10.1016/j.cub.2005.07.066 PubMed 16139209