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pUC18-mini-Tn7T-Gm-lacZ
(Plasmid #63120)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63120 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pUC18T-miniTn7T-Gm
  • Modifications to backbone
    promoterless lacZ was inserted within miniTn7 element
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    also ampicillin resistant
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC18-mini-Tn7T-Gm-lacZ was a gift from Herbert Schweizer (Addgene plasmid # 63120 ; http://n2t.net/addgene:63120 ; RRID:Addgene_63120)
  • For your References section:

    A Tn7-based broad-range bacterial cloning and expression system. Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP. Nat Methods. 2005 Jun;2(6):443-8. 10.1038/nmeth765 PubMed 15908923