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pET3CTc3A(1-135)
(Plasmid #63138)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63138 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET3C
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tc3A
  • Alt name
    Transposable element Tc3 transposase
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    426
  • Mutation
    1-135 aa bipartite domain
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3CTc3A(1-135) was a gift from Titia Sixma (Addgene plasmid # 63138 ; http://n2t.net/addgene:63138 ; RRID:Addgene_63138)
  • For your References section:

    Structural analysis of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA. Watkins S, van Pouderoyen G, Sixma TK. Nucleic Acids Res. 2004 Aug 10;32(14):4306-12. Print 2004. 10.1093/nar/gkh770 PubMed 15304566