PurposeExpression of human H2A tagged with Flag
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63560||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)394
Entrez GeneHIST2H2AC (a.k.a. H2A, H2A-GL101, H2A/q, H2AFQ)
- Promoter CMV
/ Fusion Protein
- Flag (MDYKDDDDKVPRIQ) (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3.1-Flag-H2A was a gift from Titia Sixma (Addgene plasmid # 63560 ; http://n2t.net/addgene:63560 ; RRID:Addgene_63560)
For your References section:RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling. Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen W, Marteijn JA, Sixma TK. Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005. 10.1016/j.cell.2012.08.005 PubMed 22980979