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pGKO2
(Plasmid #63617)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63617 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDHt-KS
  • Backbone manufacturer
    Kang Lab, Addgene plasmid # 63616
  • Modifications to backbone
    The modified ChGPD-HSVtk construct (as a blunted EcoRI-HindIII fragment isolated from SK1807) was cloned into a blunted SacI site of pDHt-KS (Blunt ends were generated with T4 DNA polymerase in the presence of dNTPs).
  • Vector type
    Fungal expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We removed some restriction sites from the ChGPD-HSVtk construct via site-directed mutagenesis, and at the same time, improved codons at the mutated sites based on the fungal codon usage.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGKO2 was a gift from Seogchan Kang (Addgene plasmid # 63617 ; http://n2t.net/addgene:63617 ; RRID:Addgene_63617)
  • For your References section:

    A dual selection based, targeted gene replacement tool for Magnaporthe grisea and Fusarium oxysporum. Khang CH, Park SY, Lee YH, Kang S. Fungal Genet Biol. 2005 Jun;42(6):483-92. Epub 2005 Apr 25. 10.1016/j.fgb.2005.03.004 PubMed 15893252