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pCfB2804
(Plasmid #63644)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63644 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    see publication
  • Vector type
    Yeast Expression, Cre/Lox
  • Promoter none
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

Depositor Comments

These vectors combine the advantage of efficient uracil excision reaction-based cloning (USER) and Cre-LoxP-mediated marker recycling system.
Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238

Feature list:
USER cassette 1..14
T CYC1 20..190
loxP 238..297
T URA3 298..420
Degradation tag 421..471
K.l. LEU2 472..1557
P K.l. LEU2 1558..2587
loxP 2604..2637
Consensus TY2 3' 2777..2963
pUC ori 3750..4393
AmpR 4496..5354
Consensus TY2 5' 5798..6026
T ADH1 6053..6247

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfB2804 was a gift from Irina Borodina & Jerome Maury (Addgene plasmid # 63644 ; http://n2t.net/addgene:63644 ; RRID:Addgene_63644)
  • For your References section:

    EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae. Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I. PLoS One. 2016 Mar 2;11(3):e0150394. doi: 10.1371/journal.pone.0150394. eCollection 2016. PONE-D-15-45506 [pii] PubMed 26934490