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pCfB2792
(Plasmid #63653)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63653 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    see publication
  • Total vector size (bp) 7460
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP
  • Promoter TEF1 promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see previous FEMS Yeast Research paper for more details:
http://femsyr.oxfordjournals.org/content/14/2/238

Feature list:
T CYC1 6..195
loxP 243..302
T K.l. URA3 303..425
Degradation tag 426..476
K.l. URA3 477..1277
P K.l. URA3 1278..1776
loxP 1793..1826
Consensus Ty3 3' 1966..2166
pUC ori 2947..3590
AmpR 3693..4551
Consensus Ty3 5' 4995..5228
T ADH1 5255..5449
P TEF1 5468..5887
GFP 5897..6613

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfB2792 was a gift from Irina Borodina & Jerome Maury (Addgene plasmid # 63653 ; http://n2t.net/addgene:63653 ; RRID:Addgene_63653)
  • For your References section:

    EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae. Maury J, Germann SM, Baallal Jacobsen SA, Jensen NB, Kildegaard KR, Herrgard MJ, Schneider K, Koza A, Forster J, Nielsen J, Borodina I. PLoS One. 2016 Mar 2;11(3):e0150394. doi: 10.1371/journal.pone.0150394. eCollection 2016. PONE-D-15-45506 [pii] PubMed 26934490