pYC64
(Plasmid
#63928)
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Purpose(Empty Backbone) Recipient replicative vector to construct FLAG tagged fusions
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 63928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMJ22
- Backbone size (bp) 4509
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Modifications to backboneDigested and clonning of FLAG casete as BamHI/BglII fragment
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Vector typeYeast Expression
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Selectable markersNourseothricin
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Tag
/ Fusion Protein
- FLAG
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYC64 was a gift from Irene Castano Navarro (Addgene plasmid # 63928 ; http://n2t.net/addgene:63928 ; RRID:Addgene_63928) -
For your References section:
Expression vectors for C-terminal fusions with fluorescent proteins and epitope tags in Candida glabrata. Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, Patron-Soberano A, De Las Penas A, Castano I. Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 10.1016/j.fgb.2015.04.020. 10.1016/j.fgb.2015.04.020 PubMed 25986172
Map uploaded by the depositor.
