PurposeFluorescent reporter for F-actin labeling in living cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64048||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9124
- Total vector size (bp) 10768
Modifications to backbonepLenti-hiko was digested with NheI + HpaI
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)XL10 Gold
Copy numberHigh Copy
Alt namethe first 17 amino acids of Abp140
SpeciesS. cerevisiae (budding yeast), Synthetic
Insert Size (bp)51
- Promoter Ubiquitin
/ Fusion Protein
- tdTomato (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site HpaI (not destroyed)
- 5′ sequencing primer GGCGAGTGTGTTTTGTGAAG (Common Sequencing Primers)
The Lifeact sequence was initially reported in Riedl, J. et al. Lifeact: a versatile marker to visualize F-actin. Nat Methods 5, 605–607 (2008).
Please cite the following article as follows:
Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-Lifeact-tdTomato was a gift from Weiping Han (Addgene plasmid # 64048 ; http://n2t.net/addgene:64048 ; RRID:Addgene_64048)
For your References section:Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W. Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951. 10.1038/ncomms6951 PubMed 25575350