Purpose3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64049||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9124
- Total vector size (bp) 11604
Modifications to backbonepLenti-hiko was digested with NheI + HpaI
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)XL10 Gold
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1530
Entrez GeneSlc2a4 (a.k.a. GT2, Glut-4, Glut4, twgy)
- Promoter Ubiquitin
/ Fusion Proteins
- mCherry (C terminal on insert)
- c-Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site HpaI (not destroyed)
- 5′ sequencing primer GGCGAGTGTGTTTTGTGAAG (Common Sequencing Primers)
The c-Myc sequence was inserted into the region encoding the 1st exofacial loop of mouse GLUT4 in the background of pmCherry-N1. The fragment of Myc-GLUT4-mCherry was then sub-cloned into pLenti-hiko vector via NheI + HpaI digestion.
Discrepancies between the Addgene QC sequence and the full sequence are not of functional concern.
Please cite the following article:
Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-myc-GLUT4-mCherry was a gift from Weiping Han (Addgene plasmid # 64049 ; http://n2t.net/addgene:64049 ; RRID:Addgene_64049)
For your References section:Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Lim CY, Bi X, Wu D, Kim JB, Gunning PW, Hong W, Han W. Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951. 10.1038/ncomms6951 PubMed 25575350