pLenti-FLAG-Akt2-CA
(Plasmid #64050)

• Purpose: Myristoylated-FLAG-tagged Akt2, constitutively active
• Depositing Lab: Weiping Han
• Publication: Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.

Backbone

• Vector backbone: pLenti-hiko
• Backbone size w/o insert (bp): 9124
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AKT2
• Alt name: PKB beta
• Alt name: v-akt murine thymoma viral oncogene homolog 2
• Alt name: RAC-BETA
• Species: H. sapiens (human)
• Insert Size (bp): 1446
• GenBank ID: NM_001626.5
• Entrez Gene: AKT2 (a.k.a. HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA)
• Promoter: Ubiquitin
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • MYR (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HpaI (not destroyed)
• 5′ sequencing primer: GGCGAGTGTGTTTTGTGAAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: human AKT2 sequence was derived from Addgene #9016

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MYR sequence was inserted in frame at EcoRI/NheI site into N terminal of pLenti-FLAG-hAkt2

Please cite the following article:
Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).