EcM2.1 dtolC bla::zeoR
PurposeOptimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||64054||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)This is a strain
Growth instructionszeocinR, sdsS, requires biotin supplementation in minimal media, temperature sensitive grow below 34 degrees C.
Gene/Insert namesee comments
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Escherichia coli MG1655 mutS_fs dnaG_Q576A exoX- xonA- xseA- 1255700::tolQRA Δ(ybhB-bioAB)::[λcI857 N(cro-ea59)::tetR-(bla::zeoR)] tolC-
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EcM2.1 dtolC bla::zeoR was a gift from George Church (Addgene plasmid # 64054)
For your References section:Rational optimization of tolC as a powerful dual selectable marker for genome engineering. Gregg CJ, Lajoie MJ, Napolitano MG, Mosberg JA, Goodman DB, Aach J, Isaacs FJ, Church GM. Nucleic Acids Res. 2014 Apr;42(7):4779-90. doi: 10.1093/nar/gkt1374. Epub 2014 Jan 22. 10.1093/nar/gkt1374 PubMed 24452804